CAS NO: 39450-01-6 Proteinase K

Lub luag haujlwm tseem ceeb yog txhawm rau txo qis ntau yam protein impurities hauv cov qauv, thaum tsis ua haujlwm nucleases thiab tshem tawm cov kev pheej hmoo ntawm cov protein encapsulation thiab degradation ntawm nucleic acids. Nws tuaj yeem tiv thaiv kev ncaj ncees ntawm nucleic acids. Qhov no yog qhov tseem ceeb ntawm peb tus yam ntxwv tseem ceeb ntawm substrate tshwj xeeb, tiv thaiv denaturants, thiab tsis muaj nucleic acid hydrolytic kev ua haujlwm. Nws yooj yim daws ob qhov teeb meem tseem ceeb hauv nucleic acid extraction, uas yog "protein encapsulation ntawm nucleic acids thiab nucleic acid degradation los ntawm nucleases".

Lub hom phiaj tseem ceeb yog degrade ntau yam protein impurities nyob rau hauv cov zaub mov matrix, ua txhaum lub encapsulation thiab adsorption ntawm cov proteins ntawm nucleic acids, tso lub hom phiaj nucleic acids (transgenic fragments) nyob rau hauv cov qauv, tib lub sij hawm inactivate endogenous thiab exogenous nucleases nyob rau hauv cov zaub mov, tshem tawm cov kev pheej hmoo ntawm nucleic acid degradation, txhim kho cov nucleic acid degradation, txhim kho cov nucleic acid. acid extraction, thiab xyuas kom meej lub rhiab heev thiab raug ntawm transgenic PCR / fluorescence ntau PCR nrhiav tau. Nws cov cua sov-tus neeg sawv cev tiv taus thiab dav -spectrum protein hydrolysis zog tuaj yeem hloov mus rau ntau yam khoom noj khoom haus tsis yooj yim hauv kev tshawb nrhiav zaub mov, xws li cov khoom uas muaj cov khoom sib txuam, cov ntsiab lus muaj protein ntau thiab muaj zog matrix cuam tshuam.

Lub tswv yim tseem ceeb ntawm cov proteinase K cov tshuaj tiv thaiv yog thawj zaug siv lub cev / tshuaj txoj hauv kev los rhuav tshem cov ntoo khaub lig ntom ntom - txuas cov qauv ntawm keratin, thiab tom qab ntawd hloov kho qhov pom kev zoo catalytic rau cov proteinase K, yog li txhim kho kev sib cuag ntawm cov enzyme thiab substrate. Kev siv cov proteinase K ib leeg ua rau muaj kev ua haujlwm ntawm hydrolysis tsawg heev.

1. Nucleic acid extraction: tshem tawm cov nucleases thiab lwm yam kab mob protein;
2. Biopharmaceuticals: Cov tshuaj tiv thaiv DNA, heparin, thiab lwm yam rau kev npaj cov khoom uas tsis yog-protein lom;
3. Industrial fields: tanning, cosmetic raw khoom, thiab feed processing;
4. Kev tshawb fawb lub hom phiaj: kev zom cov protein, cov khoom tseem ceeb ntawm genomic DNA / RNA extraction kits;
5. Cov enzyme no muaj qhov dav -spectrum protein hydrolysis muaj peev xwm. Nws stability thiab efficiency ua rau nws ib tug niaj hnub kuaj cov cuab yeej.

Qhov no yog ib qho tsis yog - tshwj xeeb serine protease siv rau cov protein decomposition. Nyob rau hauv lub xub ntiag ntawm sodium dodecyl sulfate (SDS) los yog urea, proteinase K yog active ntawm pH sib txawv (4 - 12), ntsev concentrations, thiab kub units.

1. Hnav lub ntsej muag plua plav lossis N95 daim npog qhov ncauj, tshuaj tiv thaiv tsom iav, thiab hnab looj tes nitrile/latex.
2. Nws yuav tsum tau nqa tawm hauv lub hood fume kom tsis txhob tsim cov plua plav.

3. Tom qab ua haujlwm, ntxuav tes kom huv si.